{"product_id":"cetrimide-agar-for-identification-of-pseudomonas-aeruginosa-500-g-acumedia-7222a","title":"Cetrimide Agar for Identification of Pseudomonas Aeruginosa, 500 g, Acumedia 7222A","description":"\u003cp\u003e\u003cstrong\u003eAcumedia® Cetrimide Agar for Identification of Pseudomonas Aeruginosa, 500 g, 7222A\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eFeatures:\u003c\/strong\u003e\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eCetrimide Agar Base with 1.3% Agar is recommended as  selective medium for isolation of Pseudomonas aeruginosa similar in composition as cited in various pharmacopoeias except that concentration of agar in this medium is 1.3%\u003c\/li\u003e\n\u003cli\u003eOriginal formula was described by King et al \u003c\/li\u003e\n\u003cli\u003eIt can also be used for determining ability of organism to produce fluorescein and pyocyanain\u003c\/li\u003e\n\u003cli\u003eCetrimide (N-acetyl-N-N,N-trimethylammaonium bromide) in  medium acts as selective agent inhibiting bacterias other than Pseudomonas aeruginosa\u003c\/li\u003e\n\u003cli\u003eIt is quarternary ammonium salt, which acts as cationic detergent that reduces surface tension in point of contact and has precipitant, complexing and denaturing effects on bacterial membrane proteins\u003c\/li\u003e\n\u003cli\u003eIt exhibits inhibitory actions on wide variety of microorganisms including Pseudomonas species other than Pseudomonas aeruginosa\u003c\/li\u003e\n\u003cli\u003eMagnesium chloride and potassium sulphate incorporated in medium enhances production of pigment pyocyanin, which is blue-green pigment, diffusing into medium which improves detection of Pseudomonas on this medium\u003c\/li\u003e\n\u003cli\u003ePresence of magnesium ions can also neutralize EDTA, if present in sample\u003c\/li\u003e\n\u003cli\u003eGelatin peptone provides essential nutrients for growth of Pseudomonas, while glycerin\/glycerol serves as slow and continuous carbon source for growing cell\u003c\/li\u003e\n\u003cli\u003eKing et al developed Medium A for enhancement of pyocyanin production by Pseudomonas\u003c\/li\u003e\n\u003cli\u003eCetrimide agar developed by Lowburry is modification of Tech Agar (Medium A) with addition of 0.1% cetrimide for selective isolation of P. aeruginosa\u003c\/li\u003e\n\u003cli\u003eLater, due to availability of highly purified cetrimide, its concentration in the medium was decreased\u003c\/li\u003e\n\u003cli\u003eIncubation was carried out at 37 degrees C for period of 18-24 hours\u003c\/li\u003e\n\u003cli\u003eP. aeruginosa can be identified due to their characteristic production of pyocyanin, a blue, water soluble, nonfluorescent phenazine pigment coupled with their colonial morphology and characteristic grape like odour of aminocetophenone\u003c\/li\u003e\n\u003cli\u003eFor isolation of P. aeruginosa, plates of cetrimide agar should be inoculated from non-selective medium such as Brain Heart infusion Broth (M210) or Soyabean Casein Digest Medium (M011)\u003c\/li\u003e\n\u003cli\u003eIf count is high, test sample can be directly inoculated onto Cetrimide Agar. P. aeruginosa colonies may appear blue, blue-green or nonpigmented\u003c\/li\u003e\n\u003cli\u003eColonies exhibiting fluorescence 250 nm and blue green pigmentation are considered as presumptive positive\u003c\/li\u003e\n\u003cli\u003eP. aeruginosa may lose its fluorescence under UV if cultures are left at room temperature for short time\u003c\/li\u003e\n\u003cli\u003eFluorescence reappears after plates are re-incubated\u003c\/li\u003e\n\u003cli\u003eGoto and Enomoto recommended that addition of nalidixic acid aids in inhibiting growth of accompanying flora\u003c\/li\u003e\n\u003cli\u003eAppearance: beige powder\u003c\/li\u003e\n\u003cli\u003epH: 7 – 7.4\u003c\/li\u003e\n\u003cli\u003eFlammability (gas, solid): non-flammable\u003c\/li\u003e\n\u003cli\u003eSolubility: soluble in water\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Others","offers":[{"title":"EA (1)","offer_id":51110774014267,"sku":"60-7222A-EA","price":125.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0921\/2041\/6571\/files\/60-7222A_bd573142-a774-4de3-a351-a3088a701884.png?v=1784700961","url":"https:\/\/preiser.com\/products\/cetrimide-agar-for-identification-of-pseudomonas-aeruginosa-500-g-acumedia-7222a","provider":"Preiser Scientific","version":"1.0","type":"link"}