Description
Lactobacilli MRS Agar is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans. MRS Agar is a medium for the cultivation and enumeration of Lactobacillus spp. Originally developed in 1960 by de Man, Rogosa & Sharpe, the medium is suitable for most lactic acid bacteria and is intended as a substitute for Tomato Juice Agar. When acidified to pH 5.4 MRS Agar can be used to enumerate Lactobacillus bulgaricus in yogurts. Nutrition is provided by a mixture of carefully selected peptones, glucose, beef & yeast extracts while Tween® 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli while inhibiting a number of other microorganism groups. Occasionally, sterilization of this medium at 121°C for 15 minutes, in some autoclaves, may cause the pH to fall outside of the specified pH limits 6.4 +/- 0.2. In these rare cases, adjustment of the medium using acetic acid or sodium hydroxide is recommended.
Specifications:
| Analyte | Lactobacillus spp. | |
| Brand | NEOGEN® Culture Media | |
| Formula | Peptone | 10 g/L |
| Yeast Extract | 5.0 g/L | |
| Beef Extract | 10.0 g/L | |
| Glucose | 20.0 g/L | |
| Dipotassium Phosphate | 2.0 g/L | |
| Sodium Acetate | 5.0 g/L | |
| Triammonium Citrate | 2.0 g/L | |
| Magnesium Sulfate | 0.2 g/L | |
| Manganese Sulfate | 0.05 g/L | |
| Tween 80 | 1.08 g/L | |
| Agar | 15.0 g/l | |
| Grams per Liter | 70.00 | |
| Liters per 500 g | 7.00 | |
| Mass | 500 g | |
| Package Dimensions | 4.60 in. x 2.90 in. x 8.60 in. | |
| Package Weight | 1.35 lb | |
| Plates per 500 g | 390 | |
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7543 Lactobacilli MRS Agar; and LabM Product: LAB093 M.R.S Agar - de Man, Rogosa and Sharpe Agar |
Specifications
| Capacity | 500 g |
|---|---|
| Material | Mixture/Ammonium Citrate Tribasic |
| D.O.T. Number | Not regulated |
| Other | Color: beige powder |
| Manufacturer | Neogen |
| Model No | 7543A |
SDS PDFs
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