Phosphate Buffer, Dilution Bottle, 99 mL (Case of 48)

Edge Biologicals® Phosphate Buffer, Dilution Bottle, 99 mL (Case of 48), EB9948WW

Features:

• Packaged in plastic flip-top containers, sealed, sterile, and ready-for-use
• Contains 99 mL of solution
• Can be used in many procedures for preparation of dilution blanks for use in examination of waters and wastewater
• Final sterilization is done by gamma radiation
• 50/case
• Prepared in accordance to the following procedure¹:

FORMULATION:

• Solution #1
  • Dibasic potassium phosphate………………34 g
  • Deionized water……………………………1000 mL
• Solution #2
  • • Magnesium chloride…………………………81.1 g
    • Deionized water……………………………1000 mL

PREPARATION:

• Sterilize both solutions by steam sterilization
• Store refrigerated
• To prepare a working solution add 1.25 mL of Solution #1 and 5 mL of solution #2 to deionized water and make up to 1 L
• Adjust pH to 7.2 ± 0.2
• Dispense into appropriate containers
• Sterilize either by autoclaving for 15 minutes at 15 lbs pressure and 121 °C (glass test tubes or glass bottles) or by gamma irradiation (plastic flip-top bottles)

QUALITY CONTROL:

• If final volume is less than 90 mL
  • Prepare pour plates of samples using 1 mL of buffer and plate count agar
  • Incubate for 72 hours at 35 °C
• If final volume is = 90 ml
  • Filter sample through a 0.22 μ filter
  • Aseptically remove the filter and place on surface of a plate count agar plate
  • Incubate at 35 °C for 72 hours

¹Greenberg, Trussell and Clesceri (ed.) 1985. Standard Methods for the Examination of Water and Wastewater, 16th ed., APHA, Washington, DC